Poplar clones of different sizes, grown on a heavy metal polluted site, are associated with microbial populations of varying composition.
Identifieur interne : 002948 ( Main/Exploration ); précédent : 002947; suivant : 002949Poplar clones of different sizes, grown on a heavy metal polluted site, are associated with microbial populations of varying composition.
Auteurs : Elisa Gamalero [Italie] ; Patrizia Cesaro ; Angela Cicatelli ; Valeria Todeschini ; Chiara Musso ; Stefano Castiglione ; Arturo Fabiani ; Guido LinguaSource :
- The Science of the total environment [ 1879-1026 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (MeSH), Betaproteobacteria (génétique), Chryseobacterium (génétique), Flavobacterium (génétique), Microbiologie du sol (MeSH), Métaux lourds (pharmacocinétique), Polluants du sol (pharmacocinétique), Populus (croissance et développement), Populus (microbiologie), Populus (métabolisme), Racines de plante (microbiologie).
- MESH :
- croissance et développement : Populus.
- génétique : Betaproteobacteria, Chryseobacterium, Flavobacterium.
- microbiologie : Populus, Racines de plante.
- métabolisme : Populus.
- pharmacocinétique : Métaux lourds, Polluants du sol.
- ARN ribosomique 16S, Microbiologie du sol.
English descriptors
- KwdEn :
- Betaproteobacteria (genetics), Chryseobacterium (genetics), Flavobacterium (genetics), Metals, Heavy (pharmacokinetics), Plant Roots (microbiology), Populus (growth & development), Populus (metabolism), Populus (microbiology), RNA, Ribosomal, 16S (MeSH), Soil Microbiology (MeSH), Soil Pollutants (pharmacokinetics).
- MESH :
- chemical , pharmacokinetics : Metals, Heavy, Soil Pollutants.
- genetics : Betaproteobacteria, Chryseobacterium, Flavobacterium.
- growth & development : Populus.
- metabolism : Populus.
- microbiology : Plant Roots, Populus.
- chemical : RNA, Ribosomal, 16S, Soil Microbiology.
Abstract
We performed a field trial to evaluate the response of different poplar clones to heavy metals. We found that poplar plants of the same clone, propagated by cuttings, had a marked variability of survival and growth in different zones of the field that were characterized by very similar physical-chemical prosperities. Since metal uptake and its accumulation by plants can be affected by soil microorganisms, we investigated soil microbial populations that were collected in proximity to the roots of large and small poplar plants. We used microbiological and molecular tools to ascertain whether bacterial strains or species were associated with large, or small poplars, and whether these were different from those present in the bulk (without plants) soil. We found that the culturable fraction of the bacteria differed in the three cases (bulk soil, small or large poplars). While some taxa were always present, two species (Chryseobacterium soldanellicola and Variovorax paradoxus) were only found in the soil where poplars (large or small) were growing, independently from the plant size. Bacterial strains of the genus Flavobacterium were prevalent in the soil with large poplar plants. The existence of different microbial populations in the bulk and in the poplar grown soils was confirmed by the DGGE profiles of the bacterial culturable fractions. Cluster analysis of the DGGE profiles highlighted the clear separation of the culturable fraction from the whole microbial community. The isolation and identification of poplar-associated bacterial strains from the culturable fraction of the microbial community provided the basis for further studies aimed at the combined use of plants and soil microorganisms in the remediation of heavy metal polluted soils.
DOI: 10.1016/j.scitotenv.2012.03.012
PubMed: 22475220
Affiliations:
Links toward previous steps (curation, corpus...)
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Michel 11, 15121, Alessandria, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Università del Piemonte Orientale Amedeo Avogadro, Dipartimento di Scienze e Innovazione Tecnologica, viale T. 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We found that poplar plants of the same clone, propagated by cuttings, had a marked variability of survival and growth in different zones of the field that were characterized by very similar physical-chemical prosperities. Since metal uptake and its accumulation by plants can be affected by soil microorganisms, we investigated soil microbial populations that were collected in proximity to the roots of large and small poplar plants. We used microbiological and molecular tools to ascertain whether bacterial strains or species were associated with large, or small poplars, and whether these were different from those present in the bulk (without plants) soil. We found that the culturable fraction of the bacteria differed in the three cases (bulk soil, small or large poplars). While some taxa were always present, two species (Chryseobacterium soldanellicola and Variovorax paradoxus) were only found in the soil where poplars (large or small) were growing, independently from the plant size. Bacterial strains of the genus Flavobacterium were prevalent in the soil with large poplar plants. The existence of different microbial populations in the bulk and in the poplar grown soils was confirmed by the DGGE profiles of the bacterial culturable fractions. Cluster analysis of the DGGE profiles highlighted the clear separation of the culturable fraction from the whole microbial community. The isolation and identification of poplar-associated bacterial strains from the culturable fraction of the microbial community provided the basis for further studies aimed at the combined use of plants and soil microorganisms in the remediation of heavy metal polluted soils.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22475220</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>30</Day></DateCompleted><DateRevised><Year>2012</Year><Month>04</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1026</ISSN><JournalIssue CitedMedium="Internet"><Volume>425</Volume><PubDate><Year>2012</Year><Month>May</Month><Day>15</Day></PubDate></JournalIssue><Title>The Science of the total environment</Title><ISOAbbreviation>Sci Total Environ</ISOAbbreviation></Journal><ArticleTitle>Poplar clones of different sizes, grown on a heavy metal polluted site, are associated with microbial populations of varying composition.</ArticleTitle><Pagination><MedlinePgn>262-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.scitotenv.2012.03.012</ELocationID><Abstract><AbstractText>We performed a field trial to evaluate the response of different poplar clones to heavy metals. We found that poplar plants of the same clone, propagated by cuttings, had a marked variability of survival and growth in different zones of the field that were characterized by very similar physical-chemical prosperities. Since metal uptake and its accumulation by plants can be affected by soil microorganisms, we investigated soil microbial populations that were collected in proximity to the roots of large and small poplar plants. We used microbiological and molecular tools to ascertain whether bacterial strains or species were associated with large, or small poplars, and whether these were different from those present in the bulk (without plants) soil. We found that the culturable fraction of the bacteria differed in the three cases (bulk soil, small or large poplars). While some taxa were always present, two species (Chryseobacterium soldanellicola and Variovorax paradoxus) were only found in the soil where poplars (large or small) were growing, independently from the plant size. Bacterial strains of the genus Flavobacterium were prevalent in the soil with large poplar plants. The existence of different microbial populations in the bulk and in the poplar grown soils was confirmed by the DGGE profiles of the bacterial culturable fractions. Cluster analysis of the DGGE profiles highlighted the clear separation of the culturable fraction from the whole microbial community. The isolation and identification of poplar-associated bacterial strains from the culturable fraction of the microbial community provided the basis for further studies aimed at the combined use of plants and soil microorganisms in the remediation of heavy metal polluted soils.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gamalero</LastName><ForeName>Elisa</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Università del Piemonte Orientale Amedeo Avogadro, Dipartimento di Scienze e Innovazione Tecnologica, viale T. Michel 11, 15121, Alessandria, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cesaro</LastName><ForeName>Patrizia</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Cicatelli</LastName><ForeName>Angela</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Todeschini</LastName><ForeName>Valeria</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Musso</LastName><ForeName>Chiara</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Castiglione</LastName><ForeName>Stefano</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fabiani</LastName><ForeName>Arturo</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Lingua</LastName><ForeName>Guido</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>04</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Sci Total Environ</MedlineTA><NlmUniqueID>0330500</NlmUniqueID><ISSNLinking>0048-9697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020562" MajorTopicYN="N">Betaproteobacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045247" MajorTopicYN="N">Chryseobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005417" MajorTopicYN="N">Flavobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000493" MajorTopicYN="Y">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" 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PubStatus="pubmed"><Year>2012</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22475220</ArticleId><ArticleId IdType="pii">S0048-9697(12)00344-0</ArticleId><ArticleId IdType="doi">10.1016/j.scitotenv.2012.03.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Castiglione, Stefano" sort="Castiglione, Stefano" uniqKey="Castiglione S" first="Stefano" last="Castiglione">Stefano Castiglione</name><name sortKey="Cesaro, Patrizia" sort="Cesaro, Patrizia" uniqKey="Cesaro P" first="Patrizia" last="Cesaro">Patrizia Cesaro</name><name sortKey="Cicatelli, Angela" sort="Cicatelli, Angela" uniqKey="Cicatelli A" first="Angela" last="Cicatelli">Angela Cicatelli</name><name sortKey="Fabiani, Arturo" sort="Fabiani, Arturo" uniqKey="Fabiani A" first="Arturo" last="Fabiani">Arturo Fabiani</name><name sortKey="Lingua, Guido" sort="Lingua, Guido" uniqKey="Lingua G" first="Guido" last="Lingua">Guido Lingua</name><name sortKey="Musso, Chiara" sort="Musso, Chiara" uniqKey="Musso C" first="Chiara" last="Musso">Chiara Musso</name><name sortKey="Todeschini, Valeria" sort="Todeschini, Valeria" uniqKey="Todeschini V" first="Valeria" last="Todeschini">Valeria Todeschini</name></noCountry><country name="Italie"><noRegion><name sortKey="Gamalero, Elisa" sort="Gamalero, Elisa" uniqKey="Gamalero E" first="Elisa" last="Gamalero">Elisa Gamalero</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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